Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe
نویسندگان
چکیده
منابع مشابه
Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe
We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reacti...
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used in this study: His6-mOrange, His6-mOrange M163K, His6-mOrange-Arg9, and His6-mCherry-Arg9. The N-terminal histidine tags were used for protein purification via immobilized metal affinity chromatography (IMAC). mOrange and mOrange M163K are proteins with pH sensitivity in a physiologically-relevant pH range. A polyarginine sequence was appended to the C-terminus of mOrange and mCherry to fa...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2014
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja506301n